The perceived ease of use of real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) technology has revolutionized life science research. Its effectiveness at amplification and ...

Scientists around the world employ quantitative real-time PCR (qPCR) to measure the amount of particular nucleic acid sequences within a sample. While this technique is rapid, sensitive, and ...

Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select ...

When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...

Large numbers of drug discovery projects involve the analysis of gene-expression levels to accurately assess target effects. When screening compounds from a library, it is common to use microarray ...

The study examined the effects of RNA sample quality and reference gene stability — two critical components of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) ...